Thermostable, Thiol Activated Keratinase from Pseudomonas aeruginosa KS-1 for Prospective Applications in Prion Decontamination
نویسندگان
چکیده
منابع مشابه
Cystic fibrosis, Pseudomonas aeruginosa, and selective decontamination.
We used an oral topical antibiotic preparation to try and prevent oropharyngeal carriage of Pseudomonas aeruginosa in patients with cystic fibrosis. Ten of 15 patients treated with a two week course of intravenous ceftazidime together with a 90 day course of an antibiotic containing gel continued to carry P aeruginosa in the oropharynx.
متن کاملBiochemical Characterization of a Thiol-Activated, Oxidation Stable Keratinase from Bacillus pumilus KS12
An extracellular keratinase from Bacillus pumilus KS12 was purified by DEAE ion exchange chromatography. It was a 45 kDa monomer as determined by SDS PAGE analysis. It was found to be an alkaline, serine protease with pH and temperature optima of 10 and 60°C, respectively. It was thiol activated with two- and eight-fold enhancement in presence of 10 mM DTT and β-mercaptoethanol, respectively. I...
متن کاملMolecular epidemiology of colistin-resistant Pseudomonas aeruginosa producing NDM-1 from hospitalized patients in Iran
Objective(s): Resistance to carbapenems is the principal reason for the continuing utilization of colistin as a last resort choice for treating the infections resulted from multidrug carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates. The assessment of antimicrobial resistance pattern, the prevalence of carbapenem-resistance determinants, and molecular epidemiology of colistin-resistan...
متن کاملEnzymatic Formulation Capable of Degrading Scrapie Prion under Mild Digestion Conditions
The prion agent is notoriously resistant to common proteases and conventional sterilisation procedures. The current methods known to destroy prion infectivity such as incineration, alkaline and thermal hydrolysis are harsh, destructive, environmentally polluting and potentially hazardous, thus limit their applications for decontamination of delicate medical and laboratory devices, remediation o...
متن کاملHigh resistance of Pseudomonas aeruginosa to paromomycin, an agent used for selective bowel decontamination (SBD)
BACKGROUND Paromomycin is used for selective bowel decontamination (SBD) in patients undergoing bone marrow transplantation in many hospitals, but there are no published resistance data for this compound in the recent medical literature. The aim of this study was to investigate the in vitro activity of paromomycin against the common intestinal bacteria E. coli and P. aeruginosa. METHODS 94 E....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Research Journal of Microbiology
سال: 2010
ISSN: 1816-4935
DOI: 10.3923/jm.2010.954.965